Differentiation and characteristics of undifferentiated mesenchymal stem cells originating from adult premolar periodontal ligaments
김성식, Kwon Dae-Woo, Im In-Sook, 손우성, 김용덕, 황대석, Holliday L Shannon, Donatelli Richard E, 전은숙,
소속 상세정보
김성식 ( Kim Seong-Sik ) - Pusan National University School of Dentistry Department of Orthodontics
( Kwon Dae-Woo ) - Pusan National University School of Dentistry Department of Orthodontics
( Im In-Sook ) - Pusan National University School of Dentistry Department of Orthodontics
손우성 ( Son Woo-Sung ) - Pusan National University School of Dentistry Department of Orthodontics
김용덕 ( Kim Yong-Deok ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
황대석 ( Hwang Dae-Seok ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
( Holliday L Shannon ) - Florida University School of Dentistry Department of Orthodontics
( Donatelli Richard E ) - Florida University School of Dentistry Department of Orthodontics
전은숙 ( Jun Eun-Sook ) - Pusan National University Hospital Biomedical Research Institute
KMID : 0361920120420060307
Abstract
Objective: The purpose of this study was to investigate the isolation and characterization of multipotent human periodontal ligament (PDL) stem cells and to assess their ability to differentiate into bone, cartilage, and adipose tissue.
Methods: PDL stem cells were isolated from 7 extracted human premolar teeth. Human PDL cells were expanded in culture, stained using anti-CD29, -CD34, -CD44, and -STRO-1 antibodies, and sorted by fluorescent activated cell sorting (FACS). Gingival fibroblasts (GFs) served as a positive control. PDL stem cells and GFs were cultured using standard conditions conducive for osteogenic, chondrogenic, or adipogenic differentiation.
Results: An average of 152.8 ± 27.6 colony-forming units was present at day 7 in cultures of PDL stem cells. At day 4, PDL stem cells exhibited a significant increase in proliferation (p < 0.05), reaching nearly double the proliferation rate of GFs. About 5.6 ± 4.5% of cells in human PDL tissues were strongly STRO-1-positive. In osteogenic cultures, calcium nodules were observed by day 21 in PDL stem cells, which showed more intense calcium staining than GF cultures. In adipogenic cultures, both cell populations showed positive Oil Red O staining by day 21. Additionally, in chondrogenic cultures, PDL stem cells expressed collagen type II by day 21.
Conclusions: The PDL contains multipotent stem cells that have the potential to differentiate into osteoblasts, chondrocytes, and adipocytes. This adult PDL stem cell population can be utilized as potential sources of PDL in tissue engineering applications.
키워드
Histochemistry;Periodontics;Bone biology
원문 및 링크아웃 정보
등재저널 정보